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Composite images (20X objective) of the same field in a microfluidic device, acquired with phase (left) or green fluorescent (right) microscopy. Rat E18 cortical neurons are healthy and extend axons to the opposite channel on DIV5. An 80μl difference in media volume prevents flow of AF488-Dextran into the grooves.

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Supervised by John H. Wolfe
Professor of Pathology and Medical Genetics in Pediatrics

Director, Walter Flato Goodman Center for Comparative Medical Genetics, School of Veterinary Medicine, University of Pennsylvania, School of Veterinary Medicine

 

Description of Research

Animal homologs of human genetic diseases are used as test systems for gene transfer by viral vectors. The approaches for transferring genes to the brain currently being investigated are ex vivo gene transfer using retrovirus and lentivius vector-modified neural stem cells transplanted to the brain and direct injection of herpesvirus, adeno-associated virus, and lentivirus vectors. The studies involve comparisons of promoters, properties of transduction for different cell types and various subregions of the brain. New methods to follow cell fate and gene expression in the live animal are being explored using MRI and PET techniques. Studies are also being directed towards better understanding of the mechanism of disease in the brain.

Rotation Projects for 2013-2014
Rotation projects are related to the molecular design and engineering of vectors, understanding the fate of vector-transferred genes in the brain, the regulation of foreign gene expression from vectors, neural stem cell biology,induced pluripotent stem cells (iPS), imaging studies, and proteomics and genomics analysis of neurodegenerative lesions. Projects can be tailored to the interest and experience of the student.

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Composite images (20X objective) of the same field in a microfluidic device, acquired with phase (left) or green fluorescent (right) microscopy. Rat E18 cortical neurons are healthy and extend axons to the opposite channel on DIV5. An 80μl difference in media volume prevents flow of AF488-Dextran into the grooves.

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