PennVet Imaging Core Facility

University of Pennsylvania School of Veterinary Medicine


The PennVet Imaging Core provides instrumentation for cellular and subcellular studies of live or fixed cells & tissues

The University of Pennsylvania Veterinary School Core Imaging Facility serves the entire Penn research community with state-of-the-art optical imaging technology. Active projects are currently focused on three general applications: (1) In vivo and intravital microscopy, including real-time imaging of pathogen infection and immune responses in the brain, intestine, skin, and secondary lymphoid organs, (2) dynamic cellular imaging, including FRET and FLIM based approaches to track the localization, interactions, and activity of signaling molecules, photo-activation to generate signaling intermediates instantaneously in situ, changes in calcium and mitochondrial function, and real time imaging of mitosis, and (3) high-quality confocal fluorescence imaging for morphological studies.

       The Penn Vet Imaging Core Facility has hired a new manager.  Dr. Gordon Ruthel received his PhD in Neuroscience from the University of Virginia in the laboratory of Dr. Gary Banker  and did his post-doctoral work in the laboratory of Dr. Peter Hollenbeck at Harvard Medical School and Purdue University.  For 11 years prior to coming to UPenn, he performed research and ran an imaging facility at the US Army Medical Institute of Infectious Diseases.  He has broad experience with a variety of widefield and confocal imaging techniques including dark field microscopy, live cell imaging with a spinning disk confocal microscope, high content imaging, multiphoton and Stimulated Emission Depletion (STED) microscopy.  Dr. Ruthel is available to provide training, assistance, and consultation.  To set up training or for assistance, Gordon can be contacted by email at or at 215 746-0471.

A list of Dr. Ruthel's previous publications can be viewed on PubMed.

Past Events: Leica Microsystems FLIM Workshop

In November 2011, in conjunction with Leica Microsystems, we hosted a 3 day mini-symposium/workshop on the use and applications of our new Leica/Picoquant Fluorescence Lifetime Imaging Microscope (FLIM).

FLIM is a method that allows real time (dynamic) visualization and quantitation of protein-protein
interactions in cells. Our new instrument is the only widely accessible instrument of it's kind in the region.

The symposium/workshops provided an opportunity to learn about FLIM and its capabilities
but also to perform measurements on relevant samples.

If you are interested in learning more about using FLIM in your research, please let me know.
Bruce Freedman
Core Scientific Director